Supplementary Figure 1: Loss of ELMO1, but not BAI1, reduces disease severity in arthritis.

a) Mice with spontaneous arthritis (K/BxN) or their healthy littermates were used to analyze engulfment machinery expression in isolated paws. b) Expression of apoptotic cell clearance components genes in total paw extracts from K/BxN mice by qRT-PCR. Each data point represents one mouse. c) Incidence of CIA in male Elmo1^+/+-DBA (n = 3, white symbols), Elmo1^+/– -DBA (n = 12, black symbols) and Elmo1^–/–-DBA (n = 6, green symbols). d) Swelling of the paws (left panel) and clinical scores (right panel) were measured in male Elmo1^+/+-DBA (n = 3, white symbols), Elmo1^+/– -DBA (n = 12, black symbols) and Elmo1^–/–-DBA (n = 5, green symbols). e) Incidence of CIA in female Elmo1^+/+-DBA (n = 5, white symbols), Elmo1^+/– -DBA (n = 8, black symbols) and Elmo1^–/–-DBA (n = 7, green symbols). f) Swelling of the paws (left panel) and clinical scores (right panel) were measured in female Elmo1^+/+-DBA (n = 5, white symbols), Elmo1^+/–-DBA (n = 8, black symbols) and Elmo1^–/–-DBA (n = 5, green symbols). g) Elmo1, Elmo2 and Elmo3 expression was analyzed by quantitative RT-PCR in the total paw extracts of Elmo1^+/– (black symbols, n = 7) and Elmo1^–/– (green symbols, n = 7) mice on day 10 after K/BxN serum injection. Each symbol represents an individual animal. h) Schematic of BAI1 binding to ELMO1-DOCK2, leading to the activation of GTPase RAC1. i) Paw swelling and clinical scores of BAI1^+/+ (n = 4, black symbols) and BAI1^–/– mice (n = 11, blue symbols) injected with 150 μl of K/BxN serum on day 0.