Supplementary Figure 3: Bacterial-challenge response and EAE are not changed in Elmo1^–/–mice.

a) Mice were challenged with LPS administered intranasally as described in the Methods, and mobilization of neutrophils was analyzed 8 hours later by flow cytometry of cells in the BAL. b) Quantification of 7-AAD^-CD11b⁺Ly6C⁺Ly6G⁺ neutrophils in the BAL of Elmo1^+/+, Elmo1^–/–, Elmo1^fl/fl and Elmo1^fl/flMrp8-Cre mice. Each symbol represents an animal. c) Elmo1^+/+ (n = 18) and Elmo1^–/– (n = 19) mice were injected with fecal contents (1.5 mg/g) intraperitoneally to induce fecal-induced peritonitis (FIP) and disease parameters were monitored as described in Methods. d) Number of Ly6G⁺ neutrophils in the peritoneum 4 hours post FIP induction. Each symbol represents an animal. e) Temperature of mice in c). f) Clinical scores of mice in c) were measured as described in Methods. g) Survival curves of mice in c). All mice alive at 72 hours post FIP induction exhibited complete recovery. h) Purified neutrophils from Elmo1^+/+ (n = 4) and Elmo1^–/– (n = 4) mice were incubated with Klebsiella pneumoniae at a 1:2 ratio for 1 hour, as described in the Methods, and bacterial killing was analyzed. Each symbol represents neutrophils from an individual animal. i) Elmo1^+/– (n = 8, black symbols) and Elmo1^–/– (n = 8, green symbols) mice were immunized to induce EAE and disease was scored over the indicated time as described in the Methods. j) Luxol Fast Blue staining of the spinal cords on day 26 after EAE induction (left panel). Demyelination was scored at four different levels of the spinal cord and composite result is shown (right panel). Each symbol represents an individual animal.