Supplementary Figure 3: Normal T cell development in ZIP7 ^P198A/P198A mice.

(a-b) Representative flow cytometry of T cell subsets in the thymus (a) and spleen (b) of WT and ZIP7^P198A/P198A (P198A-Hom) mice. Representative of 3 independent experiments (c-d) The percentage of B220⁺ B cells, naïve (CD62L⁺CD44^-), activated (CD62L^-CD44⁺) and memory (CD62L⁺CD44⁺) CD4⁺ and CD8⁺ T cells, NK cells (NK1.1⁺CD3^-), neutrophils (B220^-CD3^-Ly6g⁺CD11b⁺Ly6c⁺) and monocytes (B220^-CD3^-Ly6c⁺⁺F4/80^-) within the leukocyte populations in wild-type (closed circles) and P198A-Hom (open circles) blood (c) and spleen (d). Representative of three separate experiments; circles represent individual mice, 5 WT and 5 P198A-Hom; bars means and 95% CI, and comparisons by two-way ANOVA with Bonferroni’s correction for multiple comparison; *p<0.0001. (e) The relative proportion of thymic or splenic CD4⁺ and CD8⁺ cells, in lethally irradiated mice reconstituted for 8 weeks with 70:30 mixtures of WT or P198A-Hom CD45.2⁺ and WT CD45.1⁺ BM. Filled columns show mean percentage CD45.2⁺ cells and data are representative of 3 experiments.