Supplementary Figure 4: Transcriptomic analysis of WT and ZIP7-deficient B cells from Hardy fraction B.

B220⁺CD43⁺CD24⁺BP1^- pro-B cells were sorted flow-cytometrically and whole transcriptome analysis performed by RNAseq on 100 cell samples from WT and P198A-Hom mice. We identified genes whose transcription varied by at least two-fold between WT and P198A-Hom mice with P_adj < 0.05 (see Methods). (a-c) Heatmaps show the relative expression level of the subset of these genes that are also differentially expressed during normal B cell development: (a) during the transition from Fractions A to B (reduced, purple and raised, blue), (b) during the transition from Fractions B to C (reduced, red and raised, green) and (c) between Fractions B and D (reduced, yellow and raised, orange), based on the ImmGen database (www.immGen.org). In each heatmap, the far left column indicates the direction of change expected from ImmGen; each of the remaining columns represents one mouse and rows individual transcripts.