Supplementary Figure 4: c-Myb influences mitochondrial respiration irrespective of its impact on differentiation.

(a) Oxygen consumption rate (OCR) of CD44⁺CD62L⁺ pmel-1 Myb^+/+ and pmel-1 Myb^∆/∆ CD8⁺ T cells activated in vitro with anti-CD3 and anti-CD28 antibodies in the presence of IL-2, assessed on d5 after activation. Data are shown under basal conditions and in response to the indicated molecules (n = 5 technical replicates per time point). FCCP, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone; Eto, Etomoxir; Ant, Antimycin; Rot, Rotenone. (b, c) SRC (b) and Reduction of OCR after Etomoxir administration (c) of pmel-1 Myb^+/+ and pmel-1 Myb^∆/∆ CD8⁺ T cells generated as in a. SRC, spare respiratory capacity. (n = 3 time points per condition, each data point is the average of 5 technical replicates) (c). Data are representative of two independent experiments. Data are shown as the mean ± s.e.m.; shapes represent technical repeats. *= P < 0.05; ***= P < 0.001; (unpaired two-tailed Student’s t-test).