Supplementary Figure 1: TPEM measurements of interactions of antigen-specific and wild-type CD4+ T cells with DCs under low antigen conditions (0.01 μm PCC).
From: Quantifying in situ adaptive immune cell cognate interactions in humans
Indicated T cells (WT or 5CC7) and antigen-pulsed DCs were transferred into B10.A2 CD45.2− mice and, after 12 hours, popliteal lymph nodes were imaged by TPEM, as in Main Fig. 1. a-d, TPEM parameters plotted as mean per mouse (n=7): mean velocity (a), and mean arrest coefficient (b). The cellular interaction time for all experiments is shown in (c), and per mouse in (d). *p<0.05, **p<0.005, 2-sided Mann-Whitney U test. All center values denote the mean and error bars denote standard error of the mean. n=2 independent experiments for all panels.
