Supplementary Figure 5: B cell responses in different PPs along the small intestine. | Nature Immunology

Supplementary Figure 5: B cell responses in different PPs along the small intestine.

From: BCR affinity differentially regulates colonization of the subepithelial dome and infiltration into germinal centers within Peyer’s patches

Supplementary Figure 5

a, Diagram representing the experimental protocol presented in (b). b, B1-8hi GFP+ B cell frequency in single isolated PPs one day following cell transfer into wild-type hosts. PPs are numbered from the duodenum to the ileum. Each dot represents mean of two corresponding PPs; Data are pooled from two independent experiments with a total of two mice (n=2). c, Diagram representing the experimental protocol presented in (d). d, Transferred B1-8hi tdTomato+ B cell frequency in the GC (FAS+CD38-) and SED (CD38+CCR6+IgA+) compartments of single isolated PPs, nine days following NP-CT immunization of wild-type or antibiotics (Abx) treated hosts. Antibiotics cocktail was given to the mice in their drinking water for 2 weeks prior to immunization with NP-CT. Data are pooled from two independent experiments with 2–3 mice in each experiment (n=5). Error bars indicate s.e.m. e, Diagram representing the experimental protocol presented in Fig. 5b-f. f, Representative flow cytometry plots showing gating for NP-PE specific B cells staining for Fig. 6b. Unstained sample is used as a negative control. Two independent experiments with three mice in each experiment (n=6) showed similar results.

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