Supplementary Figure 6: Detection limit in a single co-culture.
From: Deciphering CD4+ T cell specificity using novel MHC–TCR chimeric receptors
Two hMCR2-DRB1.4-MP1103–120+ reporter clones were co-cultured with hTCC11–46 cells mixed with hTCC10–16 cells at different ratios. NFAT activation was measured after 8h (a) representative FACS dot plots, numbers indicate % NFAT+ cells and (b) statistical analysis of the results, (n = 3 co-cultures) mean ± sd are shown. One-way ANOVA analysis with multiple comparisons was done. * P < 0.02, **** P < 0.0001. nc, no co-culture.
