Supplementary Figure 5

Tlr2/Tlr4 deficiency triggers nuclear entry of Ccnd2 in a HFD-dependent manner. a-b, Representative confocal images of Ccnd2⁺Glucagon⁺ α cells of the pancreas from (a) Tlr2^+/+Tlr4^+/+ and Tlr2^−/−Tlr4^−/− littermates on 20-week HFD and (b) B6 and Tlr2^−/−Tlr4^−/− mice on 14-week HFD. Scale bars, 50 µm (upper) and 10 µm (lower). c, Representative confocal images of Ccnd2 localization in β cells of B6 and Tlr2^−/−Tlr4^−/− mice on LFD for 35 weeks. Scale bars, 50 µm (left) and 10 µm (right). d, Representative confocal images showing Cdk4⁺ Glucagon⁺ cells in islets of B6 and Tlr2^−/−Tlr4^−/− mice on 14-week HFD. Scale bars, 100 µm (left) and 10 µm (right). Representative data from 3 or 4 mice each with 2 repeats.