Supplementary Figure 2

Accumulation of β-cell, not α cell, in Tlr2^−/−Tlr4^−/− mice following HFD. a, Body weights of B6 and Tlr2^−/−Tlr4^−/− mice on HFD for 29 weeks. n = 5, 7 mice (B6, Tlr2^−/−Tlr4^−/−). b–d, Representative images showing H&E (b), Ins+ β cells (c) and Glucagon⁺ α cells (d) in pancreatic sections from B6 and Tlr2^−/−Tlr4^−/− mice on HFD for 14 or 29 weeks. Scale bars, 1 mm and 0.5 mm (d, the insets). n = 3 or 4 mice each genotype and each time point. (e) Quantitation showing number of Glucagon⁺ α cells per islet from B6 and Tlr2^−/−Tlr4^−/− mice on LFD or HFD for 14 or 29 weeks. n = 4 mice each group with n = 30, 15, and 31 islets (B6 LFD, 14-week HFD and 29-week HFD) and n = 21, 15, 31 (Tlr2^−/−Tlr4^−/− LFD, 14-week HFD and 29-week HFD). Values represent mean ± SEM. n.s., not significant, p > 0.05 by two-tailed Student’s t test.