Supplementary Figure 3: IFN-λ signaling is dispensable for effector IAV-specific CD4+ T cells responses in the lung. | Nature Immunology

Supplementary Figure 3: IFN-λ signaling is dispensable for effector IAV-specific CD4+ T cells responses in the lung.

From: Interferon-λ modulates dendritic cells to facilitate T cell immunity during infection with influenza A virus

Supplementary Figure 3: IFN-λ signaling is dispensable for effector IAV-specific CD4+ T cells responses in the lung.The alternative text for this image may have been generated using AI.

WT and Ifnlr1−/− mice were mock- or IAV-infected with PBS or 40 PFU PR8 expressing a 2W epitope, respectively. Lungs were harvested on day 9 p.i. and frequency (% of CD4+ T cells) and numbers of NP311- (a) and 2W1S- specific (b) CD4+ T cells were determined by flow cytometry. One representative of two experiments with similar results shown. Bars shown mean ± SD. n=2 (mock) or 3 (IAV) mice/group. c. On day 9 post infection, a portion of the whole lung homogenates were stimulated with media, IAV (NP311) peptide, or PMA + ionomycin for 6 hrs in the presence of brefeldin A. Following stimulation, IFN-γ, TNF, IL-17A, and IL-10 levels were determined by flow cytometry. Two pooled independent experiments are shown with n=6 (WT IAV), or 7 (Ifnlr1−/− IAV) mice/group. Bars show mean ± SEM. Statistical significance determined by one-way ANOVA followed by Tukey’s multiple comparisons test. * indicates p<0.01.

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