Supplementary Figure 4: BMDCs respond to IFN-λ. | Nature Immunology

Supplementary Figure 4: BMDCs respond to IFN-λ.

From: Interferon-λ modulates dendritic cells to facilitate T cell immunity during infection with influenza A virus

Supplementary Figure 4: BMDCs respond to IFN-λ.The alternative text for this image may have been generated using AI.

a. WT, Ifnar1−/−, or Ifnlr1−/− BMDCs were left unstimulated or stimulated with 500 ng recombinant murine IFN-λ3 or 10 ng recombinant murine IFNα2 for 1 hr. b. WT BMDCs were stimulated with increasing doses of recombinant murine IFN-λ3 for 30 min. For a and b, following stimulation cells were washed, collected in RIPA buffer, and western blots were performed for phosphorylated STAT1 (Y701), STAT1, and actin. Data shown from one of three representative experiments. c. WT BMDCs were left unstimulated or stimulated with 500 ng recombinant murine IFN-λ3 for 12 hrs. Following stimulation, relative gene expression of Ifi44, Ifit1, Isg15, and Oas3 was determined. Data from three, pooled independent experiments shown where bars represent the mean ± SEM. Statistical significance was determined using unpaired two-sided t-test. * indicates p<0.05, ** indicates p<0.01, *** indicates P<0.001.

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