Supplementary Figure 8: Ms4a4a contribution to Dectin-1-mediated control of metastatic spread.

a) Representative dot plot (left) and Mean Fluorescence Intensity (right) of WGA binding on the surface of MC38 and SL4 cells assessed by flow cytometry. Results are shown as mean ± SEM of relative MFI (fold on unstained). Three independent experiments were performed (5 independent samples for SL4, 7 independent samples for MC38). Statistical analysis by two-tailed unpaired (Mann-Whitney) Student’s t test. b, c) Number of metastatic foci in liver (b) and lungs (c) of Clec7a^-/- (open symbol) and wild-type (closed symbol) mice upon intrasplenic injection of MC38 (b) or i.v. injection of SL4 (c) cells. Results are shown as mean ± SEM. One experiment was performed (5 wild-type and 6 Ms4a4a^-/- mice). Statistical analysis by two-tailed unpaired (Mann-Whitney) Student’s t test. d) Number of metastatic foci in liver of Ms4a4a^-/- (open symbol) and wild-type (closed symbol) mice injected intrasplenic with MC38 cells upon NK cell depletion. Results are shown as mean ± SEM. Two independent experiments were performed (5 wild-type and 9 Ms4a4a^-/- mice). Statistical analysis by two-tailed unpaired (Mann-Whitney) Student’s t test and one-way ANOVA. e) Number of metastatic foci in lungs from Ms4a4a^-/- (open symbol) and wild-type (closed symbol) mice upon i.v. injection of SL4 cells. Results are shown as mean ± SEM. One experiment was performed (6 mice). Statistical analysis by two-tailed unpaired (Mann-Whitney) Student’s t test.