Supplementary Figure 2: TREM1 surface expression is induced early after MCAo in peripheral Mo/MΦ cells.
From: Peripheral TREM1 responses to brain and intestinal immunogens amplify stroke severity
TREM1 surface expression was quantified in blood at 0h before sham or MCAo, and 1h, 4.5h, 48h, and 144h (6 days) after sham surgery or MCAo. Percents of TREM1+ Mo/MΦ and PMNs in blood are shown (n=3–12 biologically independent samples per time point, mean +/- SEM; two-way ANOVA for macrophages, effects of MCAo, time, and interaction P<0.0001; Bonferroni post hoc **** P <0.0001 at 4.5h for MCAo vs sham). TREM1 surface expression was quantified in spleen at 0h before sham or MCAo, and 1h, 4.5h, 48h, and 144h (6 days) after sham surgery or after MCAo. Percents TREM1+ Mo/MΦ and PMNs in spleen are shown (n=3–12 biologically independent samples per time point, represented as mean +/- SEM; two-way ANOVA for macrophages, effect of MCAo and time, P<0.0001; effect of interaction P<0.001, Bonferroni post hoc **** P <0.0001 at 4.5h for MCAo vs sham). TREM1 MFI in blood and IL hemisphere Mo/MΦ subsets 2 days (n=5–7 biologically independent samples per group, mean +/- SEM) and 6 days (n=4–7 biologically independent samples per group, mean +/- SEM) after MCAo (Student’s two tailed t-test, ** P <0.01).
