Supplementary Figure 2: Bulk RNA-seq of ILC1a, ILC1b, ILC3b, and ILC3a subsets. | Nature Immunology

Supplementary Figure 2: Bulk RNA-seq of ILC1a, ILC1b, ILC3b, and ILC3a subsets.

From: Subsets of ILC3−ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues

Supplementary Figure 2

a, Volcano plot of differentially expressed (DE) genes between ILC1a (blue) and ILC3a (red) subsets (p < 0.05, Base Mean > 50). b, Selected transcription factors, effector molecules, and cell surface proteins that are differentially expressed between ILC3a and ILC1a (p < 0.05, Wald test). c, Heatmap of normalized expression, z-scored by row, of all differentially expressed genes (p< 0.05) between ILC1a/ILC1b and ILC3a/ILC3b. Green boxes indicate clusters of genes which form a gradient of expression between ILC1a and ILC3a. d, Venn diagram of ILC subset-signature genes. ILC1a and ILC3a signature genes were determined by selecting genes enriched in ILC1a (FC > 0, p <0.05) and ILC3a (FC < 0, p <0.05) when comparing ILC1a and ILC3a. ILC1b signature genes were determined by selecting genes enriched in ILC1b (FC < 0, p <0.05) when comparing DE genes between ILC1a and ILC1b. ILC3b signature genes were determined by selecting genes enriched in ILC3b (FC < 0, p <0.05) when comparing DE genes between ILC3a and ILC3b. Data are obtained from 3 independent biological replicates each subset.

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