Supplementary Figure 7: Expression of T-bet and Aiolos in transduced MNK3 cells. | Nature Immunology

Supplementary Figure 7: Expression of T-bet and Aiolos in transduced MNK3 cells.

From: Subsets of ILC3−ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues

Supplementary Figure 7

a, Expression of the reporter proteins GFP and mCherry indicative of T-bet and Aiolos in untransduced MNK3 cells (control) and MNK3 transduced with T-bet, or both T-bet and Aiolos. b, Expression of the transduced transcription factors by intracellular staining. One representative of 3 experiment is shown. c, Intracellular staining for IL-22 in control MNK3 cells or MNK3 transduced with Aiolos and stimulated with IL-23 and IL-1β. d, IL-22 in supernatants of stimulated cells assessed by ELISA (n=5). Data represent mean±SD. One experiment representative of 4 (c) or 2 (d) is shown.

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