Supplementary Figure 6: Representative flow plots showing TGFβ-induced signaling in peripheral blood Treg II cell subpopulation.
From: Connecting blood and intratumoral Treg cell activity in predicting future relapse in breast cancer

TGFβ (25ng/ml for 30 mins)-induced Smad2/3 phosphorylation in peripheral blood Treg II cells from newly diagnosed patients with BC (n=40) was determined by phosphoflow cytometry with anti-p-Smad2 (pS465/pS467)/p-Smad3 (pS423/pS425) antibody. Plots from relapsed patients with BC were highlighted with red box.