Supplementary Figure 1: ATP-citrate lyase (ACLY) and p300 activity are necessary for supporting pro-inflammatory gene induction during LPS activation in BMDMs.
From: Glycerol phosphate shuttle enzyme GPD2 regulates macrophage inflammatory responses
(a) ChIP-qPCR analysis of histone acetylation in Il6 and Il1b promoter regions in BMDMs stimulated with LPS for 0-1h +/- the ACLY inhibitors BMS 303141 (4 μM) or Medica16 (100 μM) (n=3). (b) qPCR analysis of Il6 and Il1b gene expression in BMDMs stimulated with LPS for 0-3h +/- 4 μM BMS 303141 or 100 μM Medica16. (c) ChIP-qPCR analysis of histone acetylation in Il6 and Il1b promoter regions in BMDMs stimulated with LPS for 0-1h +/- the p300 inhibitor C646 (p300i), 8 μM (n=3). (d) qPCR analysis of Il6 and Il1b gene expression in BMDMs stimulated with LPS for 0-3h +/- 8 μM p300i. Data are from one experiment representative of three independent experiments. Mean values shown.
