Supplementary Figure 4: The impact of rapamycin on effector molecules, transcription factors, transporters and fatty acid metabolism.
From: Quantitative analysis of T cell proteomes and environmental sensors during T cell differentiation
(a) Volcano plots showing the expression profile of effector molecules in T cells in response to mTORC1 inhibition: Granzyme B, C, D, E and N (GZMB, C, D, E and N); perforin (PRF1); interferon-γ (IFN-γ), lymphotoxin alpha (LTA); lymphotoxin beta (LTB); interleukin 2 (IL2); TNF Superfamily Member 11 (TNFSF11); TNF Superfamily Member 8 (TNFSF8); CD40 ligand (CD40LG). (b) The impact of inhibiting mTORC1 on key transcription factors in T cells – T-Box 21 (TBX21/T-bet), Proto-Oncogene C-Myc (MYC), Basic Leucine Zipper ATF-Like Transcription Factor (BATF), Interferon Regulatory Factor 4 (IRF4) and PR Domain Containing 1 (PRDM1/BLIMP1). (c) Abundance of Hypoxia Inducible Factor 1 Subunit Alpha (HIF-1α) in response to rapamycin. (d) The expression profile of glucose transporters SLC2A1 and SLC2A3 and the lactate transporter SLC16A3 in response to mTORC1 inhibition. (e) The impact of mTORC1 inhibition on proteins involved in fatty acid/sterol metabolism: Hydroxy-3-Methylglutaryl-CoA Synthase 1 (HMGCS1); Fatty Acid Desaturase 1 and 2 (FADS1 and FADS2); Stearoyl-CoA Desaturase 2/3 (SCD2/3). For a, b and e fold change calculated as +rapamycin/control using protein copy numbers. The horizontal dashed line on volcano plots indicates a P value = 0.05 (two-tailed t-test with unequal variance) while vertical dashed lines indicate a fold change of 0.67, 1 and 1.5. For a-e, n = 6 biologically independent samples for CD8+ naïve cells and 3 biologically independent samples for each of the other T cell populations. Histogram bars represent the mean +/- SD.
