Supplementary Figure 6: The impact of mTORC1 inhibition on DNA replication proteins and cell cycle protein complexes.

(a) The impact of mTORC1 inhibition on proteins implicated in DNA replication (KEGG annotation 03030 plus the addition of thymidine kinase 1 and thymidine kinase 2). Fold change calculated as +rapamycin/control using protein copy numbers. The horizontal dashed line indicates a P value = 0.05 (two-tailed t-test with unequal variance) while vertical dashed lines indicate a fold change of 0.67, 1 and 1.5. (b) Stochiometric model for cell cycle entry and progression in CD4⁺ T cells. Protein copy numbers are presented for cyclin D2 (CCND2), cyclin D3 (CCND3), cyclin dependent kinase 4 (CDK4), cyclin dependent kinase 6 (CDK6) and the cyclin dependent kinase inhibitor CDKN1B (P27). (c) The impact of rapamycin on the cyclin D/P27 model in CD4⁺ cells TCR triggered for 24 h in the presence of rapamycin, and effector T_H1 cells incubated with rapamycin for 24 h on day 5 of in vitro culture. For a, b and c, n = 3 biologically independent samples for each T cell populations. For b and c, copy numbers are rounded to the nearest thousand and are the average of biological replicates.