Supplementary Figure 4: IL-6 enhances phospho S6 levels and neutral lipid accumulation in activated T cells.
From: Fibroblastic reticular cells enhance T cell metabolism and survival via epigenetic remodeling
(a) Phospho flow cytometry of p-S6 levels in resting CD8+ T cells or those activated in whole splenocyte mixtures via anti-CD3/CD28 for 48 h or activated via anti-CD3/CD28 for 48 h in the presence of recombinant IL-6 (100ng/ml). Left panel, representative plot; right panel, summary (n = 6 biologically independent cell cultures per group) ****P < 0.0001 (two-tailed Student’s t-test) (b) Bodipy staining in CD8+ T cells either solely activated for 48 h with anti-CD3/CD28 or activated via anti-CD3/CD28 in the presence of recombinant IL-6 (100ng/ml). Left panel, representative plot; right panel, summary (n = 4 [Activated T cells] and 5 [Activated T cells + IL-6] biologically independent cell cultures) ns P = 0.0720 (two-tailed Student’s t-test) Data are a composite of two biologically independent experiments (mean ± s.d).
