Supplementary Figure 7: Generation and infection of bone marrow chimeras.

(a) Percent chimerism (following tail vein bleed) in C57BL/6J (WT) or Il6^−/− host 6-12 weeks after transfer of donor cells. Representative plots shown in a. (b) Left panel, summary of data in a; right panel, hematopoietic cell lineage reconstitution in irradiated recipients. n = 10 mice per group (mean ± s.d) (c) Frequency of CD45⁻ lymph node stromal cells. Top panel, representative plots; bottom panel, graphic display (n = pooled popliteal lymph nodes from 3 mice). (d) ICS for IL-6 in stromal populations from popliteal lymph nodes. The lymph nodes were harvested on day 8 following CFA/OVA immunization and OT-1 T cell injection. 100 μg Brefeldin A was injected intravenously (tail vein) to block protein transport 5 h prior to lymph node harvest and digest. Single cell suspensions were placed in Brefeldin A for an additional 2 h prior to ICS staining. Top panel, representative plots; bottom panel, graphic display. Data are representative of two biologically independent experiments with pooled popliteal lymph nodes from 3 wild-type C57BL/6J mice. (e) Frequency of CD44⁺, CD8⁺ T cells within the lungs of WT and Il6^−/− bone marrow chimeras. Analysis conducted in animals that successfully cleared influenza infection from Fig. 7h (n = 6 of 10 mice for WT recipients and n = 1 of 10 mice for Il6^−/− recipients) (mean ± SEM).