Extended Data Fig. 4: T cells induce Fas-caspase-8 dependent death of interacting BMDCs. | Nature Immunology

Extended Data Fig. 4: T cells induce Fas-caspase-8 dependent death of interacting BMDCs.

From: T cells instruct myeloid cells to produce inflammasome-independent IL-1β and cause autoimmunity

Extended Data Fig. 4

a, Expression of surface FasL measured by flow cytometry in in vitro polarized TH1, TH2 and TH17 cells (live, CD90.2+) cultured with WT BMDCs in the presence of anti-CD3 Ab for 1 h. Data are representative of two independent experiments. (b and c) Cell death as assayed by Zombie Yellow viability dye was measured by flow cytometry in WT, Ripk3−/−, or Ripk3−/−Casp8−/− live,CD90.2+CD11c+ BMDCs cultured with WT TH0 cells in the presence or absence of anti-CD3 Ab and neutralizing TNF Ab (20μg/mL) or FasL Ab (10μg/mL) for 6 h. Data are representative of two independent experiments.

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