Extended Data Fig. 7: Characterisation of lymphocytes in Vipr2^−/−mice.

a, Wild-type and Vipr2^−/− mice were fed ad libitum or fasted for 16 h and constitutive expression of IL-22 from CD4⁺ T cells from the small intestine was determined by flow cytometry following 4 h in vitro culture. Data show the individual responses together with the mean ± s.e.m. pooled from two independent experiments (n = 4 Wild-type mice per experiment/condition and n = 2 Vipr2^−/− mice per experiment/condition). b, Enumeration of ILC1, ILC2, total ILC3, CD4⁺ ILC3, CD4⁻ ILC3, B cells, CD8⁺ T cells and CD4⁺ T cells isolated from the small intestine of naïve wild-type and Vipr2^−/− mice. Data show the mean ± s.e.m. of one of two similar experiments (n = 8 mice per time point per experiment). a,b, Statistical significance was determined using a two-tailed unpaired Student’s t-test. *P < 0.05; **P < 0.01; NS, not significant.