Extended Data Fig. 6: Themis-deficient OTI CD8+ T cells show reduced nutrient transporter expression in response to stimulation with subthreshold affinity pMHC and cytokines.
From: T cell receptor and cytokine signal integration in CD8+ T cells is mediated by the protein Themis
(A) WT OTI lymphocytes were incubated with 50ng/ml each IL-7 and IL-12, in the presence or absence of 10μM Akt inhibitor Triciribine for 7 days. Mean +/- SEM from 3 mice/genotype from 1 experiment, data representative of 2 independent experiments. (B) Themis WT and cKO OTI lymphocytes were incubated with 50ng/ml of peptide and/or 50ng/ml of the indicated cytokine for 24h or 3 days, followed cell surface staining to detect large neutral amino acid transporter CD98 and the transferrin receptor CD71. Data from 7-9 mice/genotype from 3 independent experiments (CD98), and 6-14 mice from 4 independent experiments (CD71). WT and cKO samples were compared using unpaired t-test, with Welch’s correction if required, with ** indicating p-values from 0.001 to 0.01. Mean +/- SEM values are shown.
