Extended Data Fig. 4: Creld1 deficiency modulates cell activation and is associated with reduced cell survival.

a, Division index calculated on CFSE-labelled conventional CD4⁺ T cells stimulated for 3 days with anti-CD3/anti-CD28-conjugated beads (n = 3, unpaired two-sided t-test *p = 0.031). b, Un-normalized CFSE dilution profile of control (grey) or Creld1-deficient (red) CD4⁺ T cells after 3d-stimulation with anti-CD3/anti-CD28-conjugated beads. c, Representative flow cytometry plot for CD25, CD44, and CD69 staining in control or Creld1^cKO CD4⁺ naïve T cells after 24 h of anti-CD3/anti-CD28 stimulation. d, Exemplary plots for Bcl-2, Fas (CD95) and cleaved Caspase3 staining after 8 h ex vivo culture without stimulation. e, Exemplary histogram of flow cytometry data for Bcl-2 expression and box plot for percentage of Bcl-2^– cells in freshly isolated CD4⁺ T cells from spleen (n = 5, unpaired two-tailed t-test, *p = 0.015). f, Percentage and mean fluorescence intensity (MFI) of Fas (CD95) in freshly isolated CD4⁺ T cells from spleen (n = 4). g–i, Quantification of β-Catenin protein levels in (g) blood (n = 3) and (h) spleen (n = 3) CD4⁺ T cells measured by flow cytometry. i, β-Catenin protein levels in control and Creld1-deficient CD4⁺ T cells after 8 h ex vivo stimulation + /- TCR stimulation (n = 3). j, Representative flow cytometry plot for intracellular IFN-γ, IL-4 and IL-17 in in vitro-differentiated control and Creld1-deficient CD4⁺ T cells. k–l, In vitro CD4⁺ T cell differentiation towards (k) T_H9 (control n = 5, Creld1^cKO n = 4, One-Way ANOVA with Bonferroni post-hoc) and (l) iT_reg (control n = 5, Creld1^cKO n = 4, One-Way ANOVA with Bonferroni post-hoc) subsets. (Box plots in figure represent median value as well as 25^th-75^th percentiles; whiskers show the 5^th-95^th percentiles.).