Extended Data Fig. 5: Galectin-1 functions in a glycan-dependent manner during endotoxemia.
a, Survival of WT, Mgat5–/–, and C2gnt1–/– mice injected i.p. with 5 mg/kg LPS followed by i.p. injection of PBS or 100 µg of rGal-1 1 h later (WT LPS PBS n=6, WT LPS rGal-1 n=7, Mgat5–/– LPS PBS n=5, Mgat5–/– LPS rGal-1 n=5, C2gnt1–/– LPS PBS n=9 and C2gnt1–/– LPS rGal-1 n=11). b, Plasma amounts of ALT and LDH in WT (n=6), Mgat5–/– (n=5), and C2gnt1–/– (n=9) mice injected i.p. with 5 mg/kg LPS for 18 h. c, Galectin-1 levels in the plasma of WT (n=6), Mgat5–/– (n=5), and C2gnt1–/– (n=9) mice injected i.p. with 5 mg/kg LPS for 18 h. d, Immunoblot of CD45 that was immunoprecipitated with anti-CD45 antibody or IgG control antibody from the spleen homogenates of WT mice. Each circle represents a mouse and the horizontal lines represent mean (b,c). ns, not significant; one-way ANOVA followed by the Sidak’s post-test. Immunoblots (d) are representative of two independent experiments (d).
