Extended Data Fig. 2: Galectin-1 contributes to lethality during sepsis.
a, Plasma amounts of ALT and LDH in WT, Lgals1–/–, Casp11–/–, and Gsdmd–/– mice injected i.p. with 5 mg/kg LPS for 18 h. b, Survival of WT (n=9), Lgals1–/– (n=9), and Casp11–/– mice (n=4) injected i.p. with E. coli (5x108 CFU). c, Survival of Lgals1–/– mice injected i.p. with LPS (3 mg/kg) followed by i.p. injection of PBS (n=4), 100 µg of recombinant GFP (n=4), or 100 µg rGal-1 (n=4) 1 h later. d, Survival of Lgals1–/– mice injected i.p. with LPS (3 mg/kg) followed by i.p. injection of 100 µg of rGal-1 (n=6) or heat inactivated rGal-1 (HI rGal-1) (n=6) 1 h later. e, TNF and IL-6 secretion by WT BMDMs stimulated with 5 µM rGal-1 or 1 µg LPS for 16 h. f, Survival of WT and Casp11–/– mice injected i.p. with PBS (n=3) or 100 µg of rGal-1 (n=3). Combined data from two independent experiments are shown (b,d, and e). In (e) data are presented as mean ± SEM. In (a), each circle represents a mouse and the horizontal lines represent mean. ns, not significant (one-way ANOVA). *p < 0.05, one-way ANOVA (a); Mantel-Cox test (d).
