Extended Data Fig. 2: GCBC show high viability in culture in support of Figs. 1, 2, 4, 5, 6 and 7. | Nature Immunology

Extended Data Fig. 2: GCBC show high viability in culture in support of Figs. 1, 2, 4, 5, 6 and 7.

From: Germinal center B cells selectively oxidize fatty acids for energy while conducting minimal glycolysis

Extended Data Fig. 2

Indicated cell populations were bead purified and cultured either in RPMI media (a–c) or Seahorse XF Cell Mito Stress test media (d, e) for depicted times. Cell viability was assayed flow-cytometric staining for 7-AAD (a, d) at 120 min of culture and their viability was additionally determined at 0, 30 and 60 min utilizing Luna-FlTM automated counting with dual fluorescent microscope optics. Cells were exposed to acridine orange (AO) and propidium iodide (PI) simultaneously (b, c, e). Tabulated data are presented in (a) and (b) of 2 independent experiments depicted in red and blue. (b) shows representative images of the Lina-FlTM counter for data in (a). ns = not significant; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001 by unpaired, two-tailed t-test.

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