Extended Data Fig. 7: In vivo 13C tracing in support of Fig. 6. | Nature Immunology

Extended Data Fig. 7: In vivo 13C tracing in support of Fig. 6.

From: Germinal center B cells selectively oxidize fatty acids for energy while conducting minimal glycolysis

Extended Data Fig. 7: In vivo 13C tracing in support of Fig. 6.

LC-HRMS analysis of 13C3 lactate generated from 8 h continuous 13C6 glucose infusion using an “insulin clamp”. Mice received a primed (42.5 mU/kg)/continuous (4.5 mU/kg/min) infusion of insulin and a variable infusion of 20% glucose (50% 13C6-glucose: 50% 12C-glucose) to maintain euglycemia for 480 min. Mice were sacrificed and heart and liver were disrupted in liquid nitrogen. B cell populations were isolated as in Extended Data Fig. 1 and all samples were subjected to 13C3-lactate detection by liquid chromatography-high resolution mass spectrometry.

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