Extended Data Fig. 8: Absence of hypoxia-related gene signatures in the GCBC transcriptome in support of Fig. 7. | Nature Immunology

Extended Data Fig. 8: Absence of hypoxia-related gene signatures in the GCBC transcriptome in support of Fig. 7.

From: Germinal center B cells selectively oxidize fatty acids for energy while conducting minimal glycolysis

Extended Data Fig. 8

Depicted are quantile normalized expression values of HIF1alpha direct target genes that are involved in glycolysis (a) or not involved in glycolysis (b) and control genes that are known to be expressed or absent in GCBC (c). Significant down-regulation of hypoxia-related gene signatures in GCBC transcriptome compared to naïve B cells (left of d) and in vivo activated B cells (right of d). Data were obtained by RNA-sequencing of indicated cell populations as in Extended Data Fig. 1. Shown are averages of 3 independent RNA sequencing reactions per cell population with x-axis showing different cell populations as defined in the text (a–c). Genes are connected by lines for easier visualization and bars are means + /-SEM. Gene set enrichment plots illustrating differentially expressed genes in peak GCBCs compared to naive (left of d) and in vivo activated B cells (right of d; n = 3 per group) with respect to genes depicted in a and b. p-values were calculated using the rankSumTestWithCorrelation function in limma with t statistics.

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