Extended Data Fig. 1: Sorting scheme and principal component analysis.
From: A molecular network regulating the proinflammatory phenotype of human memory T lymphocytes
a, Sorting scheme for GM-CSF secretion assay. TEM cells were separated by sorting and further divided by secretion assay and sorting. b, Principal component analysis of RNA-seq data. Cells from nine individual donors were separated by GM-CSF secretion assay and pooled in three pools of three donors each prior to RNA-seq. This analysis showed robust results, with differences due to sample-to-sample variability accounting for only 15.45% (PC#2) of the variance, while the majority of the observed differences were due to the phenotype of the cells (65.03%, GM-CSFpos vs. neg, PC#1).
