Extended Data Fig. 6: Characterization of the effects of adjuvants in vitro and in vivo.
a, Activated BM DCs were pulsed with OVA257-264 before co-culture with naive OT-I T cells (1:2). Frequencies of CD69+ and 4-1BB+ CD8+ OT-I T cells day 5 post co-culture, data represent technical triplicates from one of three independent experiments with similar results. b, Survival (tumor area > 100mm2) from one of two independent experiments (two-tailed Mantel-Cox) of mice subcutaneously inoculated with 5 × 105 MC38 Cherry FL cells or control. Ten days later, mice were injected with poly (I:C:) (200 μg) and/or anti-4-1BB (100 μg). Subsequent poly (I:C) were administered on days 4 and 8 post initial treatment. c, E0771-OVA tumor bearing C57BL/6 WT mice were treated accordingly with OT-I FL, poly (I:C), anti-4-1BB (top; NT and FL + anti-4-1BB n = 5 mice/group, rest n = 4/group; 2-way ANOVA) or adjuvants with no T cells (bottom; n = 5 mice/group). Arrows (top) indicate adjuvants (days 5 and 8). Data points from NT, OT-I FL and OT-I FL + poly (I:C) + anti-4-1BB were included in Fig. 4c pooled data. d, Significantly upregulated genes based on GO term ‘Response to type I Interferon’ (GO: 0034340) (day 6). e, Representative CD40+CD86+ activated DCs (day 7). f–g, Absolute numbers of PMA and ionomycin stimulated, IFN-γ-producing CD8+ T cells on day 7 post adoptive transfer from 2 – 4 pooled independent experiments, symbols represent individual mice, 1-way ANOVA. h, hHer2 mice were subcutaneously injected with 1 × 106 MC38-Her2, preconditioned and treated with 1 × 107 CAR T or CAR T FL cells on days 5 and 6 post tumor inoculation (NT and FL n = 5 mice/group, CAR T n = 6, CAR T + adjuvants n = 12, FL + adjuvants n = 13, 2-way ANOVA, Sidak’s multiple comparison). Arrow indicate adjuvants on day 5 post adoptive transfer. a, c, f–h Bars represent mean ± SEM.
