Extended Data Fig. 2: Characterization of in vitro differentiated BM DCs. Related to Fig. 2. | Nature Immunology

Extended Data Fig. 2: Characterization of in vitro differentiated BM DCs. Related to Fig. 2.

From: Adoptive cellular therapy with T cells expressing the dendritic cell growth factor Flt3L drives epitope spreading and antitumor immunity

Extended Data Fig. 2: Characterization of in vitro differentiated BM DCs. Related to Fig. 2.The alternative text for this image may have been generated using AI.

a, In vitro differentiated BM DC gating strategy. b, Contour plots of BM cells differentiated with (FL-DCs) or without (GM-DCs) exogenous Flt3L (20 ng/mL). c, IFN-γ and TNF levels in supernatants of 4 × 104 naive OT-I T cells co-cultured for 5 days with OVA257-264 peptide (0.1 μg/mL) pulsed GM- or FL-DCs at a 2:1 ratio. Where indicated, DCs were activated with poly (I:C) (25 μg/mL) for 24 hours prior to co-culture. Bars represent mean ± SEM of technical triplicates from one of three independent experiments with similar results. d, Schematic illustration of OT-I T cell transduction and preconditioning with cytokines IL-7 and IL-15 (IL-7/15).

Source data

Back to article page