Extended Data Fig. 5: Recombinant IL-18 treatment rescues Cyld−/−Il18−/− mice but not Cyld−/− mice from severe colitis.
From: Deubiquitination of NLRP6 inflammasome by Cyld critically regulates intestinal inflammation
(a) Body weight change (***P<0.0001) and (b) diarrhea score (***P<0.0001) of wild-type, Cyld−/−, Il18−/−, and Cyld−/−Il18−/− mice (n=8 mice per group) infected (inf) with C. rodentium. (c), Body weight change (***P<0.0001), (d) diarrhea score (***P<0.0001), and (e) colon length (***P<0.0001, **P=0.0039, ***P<0.0001, ***P<0.0001) of wild-type, Cyld−/−, Il18−/− and Cyld−/−Il18−/− mice (n=8 mice per group) infected (inf) with C. rodentium and along with rIL-18. (f) H&E-stained colonic sections from C. rodentium–infected and rIL-18 -treated wild-type, Cyld−/−, Il18−/− and Cyld−/−Il18−/− mice (scale bars, 50 µm) and g, histology scores of the sections (n=8 mice per group, ***P=0.0002, ***P=0.0001). Statistics are mean ± SD, with P values determined by Student’s t test (two tail). Data are from one experiment representative of three independent experiments with similar results.
