Extended Data Fig. 1: Deletion of Tet genes in B cells.
From: Tet2 and Tet3 in B cells are required to repress CD86 and prevent autoimmunity
Schematic diagrams of floxed and deletion alleles of Tet2 and Tet3 genes with primer sets for detection of genomic deletion (Upper panel). Genomic PCR for detection of floxed and deletion alleles with genomic DNA purified from T (TCR-b+) and B (CD19+) cells isolated from Tet2 f/f, Tet3 f/f, CD19 Cre-/- (Cre-, n=4) and Tet2 f/f, Tet3 f/f, CD19 Cre+/- (Cre+, n=4) mice (Lower left panels). mRNA expression of Tet genes in T and B cells isolated from bDKO mice measured by quantitative PCR (Lower right panels). β-actin was used for internal control. Data are mean ± SD.
