Extended Data Fig. 3: Transcription factor expression in mouse and human memory B cells.

a, Heatmap of each cell’s (column) expression of select TFs per cluster (rows). Log-normalized expression scaled for each gene. Cluster name displayed below. b, Heatmap of TF expression from bulk RNA-seq of splenic GC B cells, PreMem B cells, and MBCs at day 11 post LCMV infection. c, TF expression in human naïve, GC, and MBCs as determined using datasets compiled by Genevestigator. 3 samples were included in each dataset. Level of expression is a unitless value on a log2 scale computed following normalization and scaling of raw data in order to allow comparison of expression of given gene between samples. Statistical analyses were performed using the two-sided Holm-Sidak multiple comparisons test (****, p < 0.0001). Scatter plots indicate mean (middle line) with error bars indicating standard error mean. d, Representative FACS plots of CXCR3⁺ cells (left) among Thy1.1⁺BFP⁺ MBCs (B220⁺IgD^loGL7⁻CD38⁺GFP⁺) at day 30 post LCMV infection in mice containing cells transduced with sgRNAs targeting a control nonexpressed gene and CXCR3. Percentage of CXCR3⁺ cells among Thy1.1⁺, BFP⁺, Thy1.1⁺BFP⁺, and Thy1.1^-BFP⁻ MBCs (right). Data are pooled from 4 independent experiments with at least 4 mice per group. Scatter plots indicate mean (middle line) with error bars indicating standard error mean. e, Representative FACS plots of CD38⁺ cells (left) among Thy1.1⁺BFP⁺ B220⁺IgD^loGFP⁺ cells at day 30 post LCMV infection in mice containing cells transduced with sgRNAs targeting a control nonexpressed gene and CD38. Percentage of CD38⁺ cells among Thy1.1⁺, BFP⁺, Thy1.1⁺BFP⁺, and Thy1.1^-BFP⁻ B220⁺IgD^loGFP⁺ (right). Data are pooled from 4 independent experiments with at least 4 mice per group. Scatter plots indicate mean (middle line) with error bars indicating standard error mean. f, Ratio of MBCs to GC B cells (B220⁺IgD^loGL7⁺CD95⁺GFP⁺) among sgRNA 1-Thy1.1⁺sgRNA 2-BFP⁺ B cells in the experiment described in Fig. 3c. Data are pooled from 6 independent experiments with at least 3 mice per group. Statistical analyses were performed using the ordinary one-way ANOVA with two-sided Dunnett multiple comparison testing. Scatter plots indicate mean (middle line) with error bars indicating standard error mean.