Extended Data Fig. 3: Increased turnover of glycolytic enzymes following activation.
From: Dynamics in protein translation sustaining T cell preparedness
(a) Naïve or 6h-activated T cells were analyzed on a Seahorse analyzer. Shown is the Extra Cellular Acidification Rate (ECAR), which is a measure of the glycolytic rate. n = 6 from three donors. Bars represent the S.E.M (b) Gene ontology analysis of the proteome of naïve T cells. Glycolytic proteins (blue) contributed 11% to the cytosolic protein mass. (c) Fraction of newly synthetized (heavily labeled) glycolytic proteins after a 12-hours pulse in naïve or 12h-activated T cells. n = 3 from three different donors. Box plot elements are defined as in Fig. 2b.
