Extended Data Fig. 1: Viability of resting T cells in culture.
From: Dynamics in protein translation sustaining T cell preparedness
(a) Protein mass of naïve T cells does not change after 72 h of culturing. Proteomes of T cells that were either analyzed immediately after isolation or after 72 h of culturing were analyzed by LC-MS. Protein content was estimated using the proteome ruler approach59. n = 5 for freshly isolated and n = 2 for 72h-cultured T cells from different donors. Bars represent the S.E.M (b) FACS-purified naïve and memory T cells were cultured in complete medium without the addition of growth factors. To measure cell viability, T cells were stained with Annexin-V-FITC directly after sorting or after 24 h and 96 h of culturing and then analyzed by flow cytometry. n = 4, Four independent experiments with T cells from two different donors. Bars represent the S.E.M. (c) FACS-purified naïve T cells were cultured for 24 h in complete medium containing either DMSO, 50 µg/ml CHX or 50 µg/ml CHX together with 10 µM bortezomib (CHX + PS). n = 8 from three different donors. Bars represent the S.E.M.
