Extended Data Fig. 2: Accumulation of non-hemolytic catalase-positive cocci in the dermis of tape-stripped skin.
From: The commensal skin microbiota triggers type I IFN–dependent innate repair responses in injured skin
a FISH staining of bacterial 16S rRNA of uninjured (top) and tape-stripped (bottom) murine skin. Reverse probes for 16S rRNA were used as controls. Scale bars are shown on images. b, Colorimetric Gram staining of uninjured and injured skin, showing the presence of cocci in the dermis of stripped skin but not in uninjured skin. a-c, images are representative of 5 different mice. c, Photographs of a blood-agar plate showing non-hemolytic bacterial colonies grown overnight from tape-stripped skin of three mice (top line). One colony per mouse was tested for catalase activity by adding 3% H2O2 . Absence of gas bubbles indicates catalase negativity (middle line). Gram staining of bacteria harvested from the injured skin analyzed by light microscopy (bottom line).
