Extended Data Fig. 4: Only CXCL10 skin injection induces Ifna, but all CXCL9, CXCL10, and CXCL11 kill bacteria, bind DNA, and induce type I IFN production in pDC.
From: The commensal skin microbiota triggers type I IFN–dependent innate repair responses in injured skin
a, Expression of Ifna2 in the skin of mice 6 hours after intradermal injection of CXCL9, CXCL10, CXCL11, chemerin, or control saline. Data represent the mean + s.e.m. n = 4. *P<0.01 (one-way ANOVA followed by Tukey’s multiple comparisons test). b, Percentages of viable SytoGreen+ SytoxOrange- bacteria (Staphylococcus epidermidis) incubated for 24 hours with increasing concentrations of CXCL9, CXCL10, CXCL11, or Chemerin assessed by flow cytometry. Data represent the mean + sd of triplicates. c, Fluorimetric quantification of DNA staining by PicoGreen® dye after mixing of bacterial DNA with CXCL9, CXCL10, CXCL11, or Chemerin. A.U., arbitrary units. Data represent the mean + sd of triplicates. * P<0.05 (one-way ANOVA followed by Dunnett’s multiple comparisons test). d, Amounts of IFN-α produced by human blood-isolated pDC stimulated for 24 hours with purified DNA from bacteria in the presence or not of CXCL9, CXCL10, CXCL11, or Chemerin. Data represent the mean + sd. n=3. **** P<0.0001 (two-way ANOVA followed by Sidak’s multiple comparisons test).
