Extended Data Fig. 5: Organ-specific transcriptome features.
From: Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection
a, Heatmap showing row-scaled expression of the ten highest DEGs per organ for each averaged organ profile. b, As in a. but for each G5 subpopulation between PB and SP. KEGG analysis of DEGs for each G5 in these two organs. Left: selected KEGG terms with Benjamini-Hochberg-corrected P-values < 0.05 are shown. c, The percentages of each neutrophil subpopulation in the BM and PB calculated based on the scRNA-seq data (Fig.1c). d, The absolute numbers of each neutrophil subpopulation in the BM and PB calculated based on the percentage in (c) and predicted total BM neutrophil count32,33. (e-j), The transcriptome feature of the three G5 populations in the BM, PB, and SP. e, Heatmap showing row-scaled expression of DEGs across organs in each G5 cluster. (f-h), GO analysis of DEGs across organs. Selected GO terms with Benjamini-Hochberg-corrected P-values < 0.05 (one-sided Fisher’s exact test) are shown. i, Violin plots of maturation score and apoptosis score for each G5 neutrophil subpopulation across organs. j, Proportions of apoptotic cells in each G5 neutrophil subpopulation across organs.
