Extended Data Fig. 6: β-catenin is required for Rspondin3-induced macrophage phenotype transition. | Nature Immunology

Extended Data Fig. 6: β-catenin is required for Rspondin3-induced macrophage phenotype transition.

From: The angiocrine Rspondin3 instructs interstitial macrophage transition via metabolic–epigenetic reprogramming and resolves inflammatory injury

Extended Data Fig. 6

Levels of anti-inflammatory marker genes (Mrc1, Arg1, Retnla, Chil3) and pro-inflammatory marker genes (Cd86, Il1b, Tnf, Cxcl1) measured by qPCR in BMDMs from Ctnnb1Mφ-/- and WT mice at basal condition, or treated with Rspondin3, LPS alone or combination of both for 24h; Data are representative of three independent experiments with n=3 samples per group (mean ± sd); Statistical significance was determined by two-way ANOVA with Tukey’s multiple comparisons test using GraphPad Prism with individual P values (left to right) are: Mrc1 (ns P>0.9999, ****P<0.0001, ns P>0.9999, ****P<0.0001), Arg1 (ns P>0.9999, ****P<0.0001, ns P>0.9999, ****P<0.0001), Retnla (ns P>0.9999, ****P<0.0001, ns P=0.9716, ****P<0.0001), Chil3 (ns P=0.9998, ****P<0.0001, ns P=0.8135, ****P<0.0001), Cd86 (ns P>0.9999, ns P=0.9878, ****P<0.0001, ****P<0.0001), Il1b (ns P=0.9999, ns P=0.9946, ****P<0.0001, ****P<0.0001), Tnf (ns P=0.9854, ns P=0.9426, ****P<0.0001, ****P<0.0001), Cxcl1 (ns P>0.9999, ns P=0.9975, ****P<0.0001, ****P<0.0001).

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