Fig. 1: SARS-CoV-2 infection in K18-hACE2 mice.
a–e, Eight- to nine-week-old male and female K18-hACE2-transgenic mice were inoculated via the intranasal route with 2.5 × 104 p.f.u. SARS-CoV-2. a, Weight change was monitored (two experiments; n = 10; two-way ANOVA: ***P = 0.0002; ****P < 0.0001; symbols represent means ± s.e.m.). b,c, Viral burden in the lungs was analyzed at 2, 4 and 7 dpi by plaque assay for infectious virus (b) and RT-qPCR for viral RNA levels (c). For b, one-way ANOVA with Dunnett’s test was used to determine significance (****P < 0.0001; NS, not significant). d, Viral RNA levels in the indicated tissues (heart, brain, kidney, spleen, serum and gastrointestinal tract) at 2, 4 and 7 dpi, as measured by RT-qPCR. e, Viral burden in the brains, as measured by plaque assay. For b–e, two experiments were performed (n = 10). Bars represent means and dotted horizontal lines indicate the limit of detection.
