Fig. 3: Functional consequences of SARS-CoV-2 infection in K18-hACE2 mice.
a–k, Clinical chemistry and hematological parameters (measures of sodium (a), potassium (b), chloride (c), anion gap (d), bicarbonate (e), blood urea nitrogen (f), glucose (g), calcium (h), hematocrit (i), hemoglobin (j) and prothrombin time (k)) from the peripheral blood of mock-treated or SARS-CoV-2-infected animals at 2, 4 and 7 dpi (two independent experiments; n = 15 for naive mice; n = 12 for 2 dpi; n = 15 for 4 dpi; n = 15 for 7 dpi; one-way ANOVA with Dunnett’s test; P values: ***P = 0.0009 (c); *P = 0.0237 and ****P = 0.0001 (d); ****P < 0.0001 (e,i–k)). l, Serum D-dimer levels of mock-treated or SARS-CoV-2-infected animals at 2, 4 and 7 dpi (two independent experiments; n = 5 for naive mice; n = 5 for 2 dpi; n = 6 for 4 dpi; n = 5 for 7 dpi; one-way ANOVA with Dunnett’s test; *P = 0.0246). m. Results of a treadmill performance test, as measured by the distance traveled in 6 min (two independent experiments; n = 6 for naive mice; n = 6 for 2 dpi; n = 8 for 4 dpi; n = 6 for 7 dpi; two-way ANOVA; ****P < 0.0001). n–w, Respiratory mechanics parameters (respiratory system resistance (n), respiratory system elastance (o), inspiratory capacity (p), quasistatic compliance (q), pressure–volume loops (r), shape parameter K (s), hysteresis area (t), Newtonian resistance (u), tissue damping (v) and tissue elastance (w)) from the lung function assessment in mock-treated or SARS-CoV-2-infected male and female mice at 2, 4 and 7 dpi. Individual results with group means from two independent experiments are shown (n = 6 for naive mice; n = 6 for 2 dpi; n = 8 for 4 dpi; n = 6 for 7 dpi; one-way ANOVA with Dunnett’s test; P values: ***P = 0.0001 (n); ***P = 0.0002 (o); **P = 0.0024 (p); ***P = 0.0008 (q); **P = 0.0015 (s); **P = 0.0044 (t); ***P = 0.0002 (v); ***P = 0.0003 (w)). Bars represent means and dotted horizontal lines indicate the mean value of the mock-infected group. Asterisks indicate statistical significance as compared with mock infection. In m and r, symbols represent means ± s.e.m.
