Extended Data Fig. 4: Phenotypic comparison of Treg and Teff TF KO cells.
From: Functional CRISPR dissection of gene networks controlling human regulatory T cell identity
We selected a subset of candidate Treg TFs. In addition, here we selected additional candidate TFs that are preferentially expressed in conventional CD4+T cell subsets (referred to here as Teff cells) based on RNA-seq data (Roadmap Epigenomics Project15). Corresponding H3K27ac and RNA-seq data (Treg, Tnaive, TH, TH stim and TH17 stim) are shown on the left for each tested TF. On the right: Representative personality plots of TF KO Treg cells and TF KO Teff cells after IL-12 stimulation of one of two donors tested. Note: Treg cells from this blood donor had distinct cytokine responses compared to those included in the larger arrayed TF KO screen experiments. IRF8 and MYBL1 were targeted with 3 independent gRNAs, while the other TFs were targeted with a previously validated gRNA (includes data of 2 human donors and 1 technical replicate for each condition). crRNA sequences and editing efficiencies are shown in Supplementary Table 3. Note: changes in IL-4 regulation helped to distinguish effects of TF KO in Treg versus Teff cells in these experiments and IL-4 is therefore included in these personality plots.
