Extended Data Fig. 7: Functional assessment of HIVEP2 KO Treg cells in a humanized mouse model of GVHD.
From: Functional CRISPR dissection of gene networks controlling human regulatory T cell identity
PBMC and Treg cells from 2 human blood donors were injected in a 2:1 ratio into NSG mice. Survival rate (a) and weight changes (b) were monitored over 40 days. PBMC alone (n = 5), PBMC+AAVS1 KO Treg cells (targeted a control safe harbour locus; n = 3), PBMC+FOXP3 KO Treg cells (n = 2), PBMC+HIVEP2 KO Treg cells (n = 3). The mice were all male age matched from three litters and randomized to different experimental groups. (c) Detailed information about the number of mice used and their survival time. Experiment 1 and 2 refers to the two different human blood donors. crRNA sequences and editing efficiencies are shown in Supplementary Table 3.
