Extended Data Fig. 2: Riok2 haploinsufficient mice display anemia and myeloproliferation.
From: Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias
a, Gating strategy used for the identification of erythroid progenitor/ precursor cells in the BM. b, Number of erythroid progenitor populations among viable BM cells in Riok2f/+Vav1cre mice and Riok2+/+Vav1cre controls. n = 5/group. c, Cell cycle analysis of erythroid progenitor/ precursor cells from Riok2 haploinsufficient mice in comparison to Vav1cre controls. n = 5 mice/group. d, Cdkn1a mRNA expression by qRT-PCR in erythroid progenitors from Riok2 haploinsufficient mice and Vav1cre controls. n = 3 mice/group. e, Kaplan-Meier survival curve for Riok2 haploinsufficient mice and Vav1cre controls subjected to lethal dose of PhZ. f, Number of RIII and RIV erythroid precursor populations among viable BM cells in Riok2f/+Vav1cre mice and Riok2+/+Vav1cre controls day 6 after PhZ treatment. n = 4/group. g, PB RBC numbers, Hb, and HCT in mice transplanted with either Riok2 haploinsufficient mice or Vav1cre BM cells. n = 5 mice/group. h, PB RBC numbers, Hb, and HCT in mice with tamoxifen-inducible deletion of Riok2. Tamoxifen administered on days 3 – 7. n = 8 and 7 for Riok2+/+Ert2cre and Riok2f/+Ert2cre mice, respectively. i, Representative flow cytometry plots showing frequency of monocytes (CD11b+Ly6G−Ly6Chi) and neutrophils (CD11b+Ly6G+) in the PB of Riok2f/+Vav1cre and Riok2+/+Vav1cre mice. j, Representative flow cytometry plots showing Ki-67+ GMPs in the BM of Riok2f/+Vav1cre and Riok2+/+Vav1cre mice. k, Number of CFU-GM colonies in MethoCult from Lin−Sca-1+c-kit+ BM cells from Riok2f/+Vav1cre mice (n = 5) and Riok2+/+Vav1cre controls (n = 4) after a 7-day culture period. n = 4-5/group. Multiple unpaired two-tailed t-tests with Holm-Sidak method (b, c), unpaired two-tailed t-test (d, f, g, k), log-rank test (e), and 2-way ANOVA with Sidak’s correction for multiple comparisons (h) used to calculate statistical significance. Data are shown as mean ± s.e.m and are representative of two (b to k) independent experiments. * p<0.05, ** p<0.01, *** p<0.001.
