Extended Data Fig. 5: Expression of lineage-associated T cell cytokines is comparable between Riok2 haploinsufficient and sufficient T cells.
From: Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias
a-g, Concentration of IL-2 (a), IFN-γ (b), IL-4 (c), IL-5 (d), IL-13 (e), IL-17A (f) and frequency of Foxp3+ cells (g) from in vitro polarized T cells of the indicated genotypes. n = 3 mice/group. h-i) Number of IL-22+ NKT cells (H) and ILCs (I) in the spleens of Riok2f/+Vav1cre mice and Riok2+/+Vav1cre controls (n = 4/group). j, Frequency of IL_23p19+ DCs in Riok2f/+Vav1cre mice and Riok2+/+Vav1cre controls. n = 4 mice/group. k, PB RBC numbers, Hb, and HCT in Riok2f/fCd4cre mice (n = 3) in comparison to Riok2+/+Cd4cre controls (n = 5). l, Secreted IL-22 from in vitro polarized TH22 cells from Rps14 haploinsufficient mice and Vav1cre controls. n = 4 mice/group. m, Secreted IL-22 from in vitro polarized TH22 cells from ApcMin mice and littermate controls. n = 4 mice/group. n, Viable cells (expressed as percentage of total cells in culture) for the indicated treatments assessed by flow cytometry. n = 5 mice/group. Data are shown as mean ± s.e.m and are representative of two (a to n) independent experiments. Unpaired two-tailed t-test (a to n) used to calculate statistical significance. * p < 0.05, ** p < 0.01.
