Extended Data Fig. 5: Lung CD8 T cells during lethal challenge with influenza A virus.
From: TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming
a, Percent of influenza A specific CD8+ T cells in the lungs of WT→WT and Tap⎯/⎯→WT mice at days 3 and 5 post lethal challenge with 75 p.f.u. influenza A PR8 virus. Data show two different H-2Dd tetramers loaded with either a polymerase acidic protein epitope (SSLENFRAYV) or a nucleoprotein epitope (ASNENMETM). b, Representative FACS plot and % CD8+CD44+ cells in the lung of naïve chimeric mice. c, Flow cytometry plots showing CD8+ T cells in the lungs or spleens of WT mice on days 1 and 2 following injection with anti-CD8α antibody (clone 2.43) intranasally (i.n., 200µg), intraperitoneally (i.p., 100µg), or via both routes (i.p.+i.n.). Percent of CD3+CD8β+ T cells remaining are indicated in the blue gates. The mean ± SEM are presented and each symbol represents a mouse. N.S.= non statistically significant (P>0.05) using an unpaired two-tailed t-test.
